Journal
MICROCHIMICA ACTA
Volume 182, Issue 7-8, Pages 1431-1437Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-015-1469-9
Keywords
Graphene quantum dots; Urea; Detection; Urease
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Funding
- National Natural Science Foundation of China [21375003, 21175003, 21303003]
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We report on a nanoparticle-based fluorescent sensing scheme for urea. It is based on the finding that graphene quantum dots (GQDs) display pH-sensitive green fluorescence if photoexcited at 460 nm. Fluorescence is gradually quenched due to an increase in the local pH value as a result of the hydrolysis of urea as catalyzed by urease. The effect was used to quantify urea in the 0.1-100 mM concentration range, with a limit of detection of 0.01 mM. The method was successfully applied to the determination of urea in human serum samples. The method is simple, effective, and therefore holds promise as a platform for sensing urea in blood.
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