Journal
MICROCHIMICA ACTA
Volume 182, Issue 9-10, Pages 1709-1714Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-015-1492-x
Keywords
Fumonisin; Biosensor; Electrochemical impedance spectroscopy (EIS); Electrodeposited gold nanoparticles; Aptamer
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Funding
- National S&T Support Program of China [2012BAK08B01]
- S&T Supporting Project of Jiangsu Province [BE2011621]
- [JUSRP51309A]
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We are presenting an aptasensor for the sensitive determination of fumonisin B1 (FB-1) via electrochemical impedance spectroscopy (EIS) and applying aptamer-based biorecognition. A thiolated aptamer for FB-1 was anchored onto the surface of gold nanoparticles (AuNPs) on a glassy carbon electrode. A significant increase in resistance (R-et) is found on interaction with FB-1 in the 0.1 nM to 100 mu M concentration range, and the detection limit is as low as 2 pM. The assay was applied to determine FB-1 in spiked maize samples and gave recovery rates ranging from 91 to 105 %. The results demonstrate this method to present new possibilities in the application of aptamers in food safety analysis.
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