Journal
MICROCHIMICA ACTA
Volume 182, Issue 11-12, Pages 1933-1939Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-015-1526-4
Keywords
CuInS2 quantum dots; Near-infrared; Thrombin; Aptamer; Fluorescence
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Funding
- National Natural Science Foundation of China [21075050, 21275063]
- science and technology development project of Jilin province, China [20110334]
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We describe a near-infrared (NIR) fluorescent thrombin assay using a thrombin-binding aptamer (TBA) and Zn(II)-activated CuInS2 quantum dots (Q-dots). The fluorescence of Zn(II)-activated Q-dots is quenched by the TBA via photoinduced electron transfer, but if thrombin is added, it will bind to TBA to form G-quadruplexes and the Q-dots are released. As a result, the fluorescence intensity of the system is restored. This effect was exploited to design an assay for thrombin whose calibration plot, under optimum conditions, is linear in the 0.034 to 102 nmol L-1 concentration range, with a 12 pmol L-1 detection limit. The method is fairly simple, fast, and due to its picomolar detection limits holds great potential in the diagnosis of diseases associated with coagulation abnormalities and certain kinds of cancer.
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