Journal
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
Volume 79, Issue 3, Pages 347-372Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/MMBR.00006-15
Keywords
-
Categories
Funding
- Canadian Cancer Society (CCS) [702834]
- Canadian Institutes of Health Research (CIHR) [MOP-115127]
- Medical Research Council UK
- MRC [MC_PC_U127527202] Funding Source: UKRI
- Medical Research Council [MC_PC_U127527202] Funding Source: researchfish
Ask authors/readers for more resources
In humans, nearly two meters of genomic material must be folded to fit inside each micrometer-scale cell nucleus while remaining accessible for gene transcription, DNA replication, and DNA repair. This fact highlights the need for mechanisms governing genome organization during any activity and to maintain the physical organization of chromosomes at all times. Insight into the functions and three-dimensional structures of genomes comes mostly from the application of visual techniques such as fluorescence in situ hybridization (FISH) and molecular approaches including chromosome conformation capture (3C) technologies. Recent developments in both types of approaches now offer the possibility of exploring the folded state of an entire genome and maybe even the identification of how complex molecular machines govern its shape. In this review, we present key methodologies used to study genome organization and discuss what they reveal about chromosome conformation as it relates to transcription regulation across genomic scales in mammals.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available