Journal
INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY
Volume 33, Issue 3, Pages 309-316Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/PGP.0b013e31829c673b
Keywords
BRCA2; gamma-H2AX; BRCA1; RRSO; p53
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Funding
- Tampere University Hospital [9L098]
- Finnish Medical Foundation
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Mutations in BRCA1 and BRCA2 genes confer an increased lifetime risk for breast and ovarian cancer. Increased lifetime ovarian cancer risk among BRCA1/BRCA2 mutation carriers can be substantially decreased by risk-reducing salpingo-oophorectomy (RRSO), which also provides material for molecular research on early pathogenesis of serous ovarian cancer. RRSO studies have suggested fallopian tube as a primary site of serous high-grade ovarian cancer. In this study, the nuclear expression levels of gamma-H2AX and p53 using immunohistochemical (IHC) study was quantitatively assessed in ovarian and fallopian tube epithelium derived from RRSOs in 29 BRCA1 and BRCA2 mutation carriers and in 1 patient with a strong family history of breast and ovarian cancer but showing an unknown BRCA status. Both p53 and gamma-H2AX nuclear staining levels were significantly higher in BRCA1/2 mutation-positive fallopian tube epithelium compared with the control fallopian tube epithelium (P < 0.006 and P=0.011, respectively). Nuclear expression levels of p53 and gamma-H2AX were similar between the BRCA1/2 mutation-positive ovarian epithelium and controls. Both gamma-H2AX and p53 showed significantly higher nuclear expression levels in BRCA1/2 mutation-positive fallopian tube epithelium compared with BRCA1/2 mutation-positive ovarian epithelium (P < 0.0001 and P < 0.0001, respectively). BRCA1/2 mutation-positive fallopian tube epithelium showed a positive correlation between the gamma-H2AX and p53 nuclear expression levels (Pearson r=0.508, P=0.003). Our results of quantitative nuclear p53 and gamma-H2AX expression levels in ovarian and fallopian tube epithelium derived from RRSO in high-risk patients support the previously suggested role of fallopian tube epithelium serving as a possible site of initial serous ovarian carcinogenesis.
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