Journal
MICROBIOLOGICAL RESEARCH
Volume 178, Issue -, Pages 51-58Publisher
ELSEVIER GMBH
DOI: 10.1016/j.micres.2015.06.002
Keywords
Fis1; Mitochondrion; Conidiation; Pathogenicity; Magnaporthe oryzae
Categories
Funding
- National Basic Research Program of China [2012CB114002]
- Natural Science Foundation of China [31370171, 31371891]
- Fundamental Research Funds for the Central Universities, Pakistan
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The mitochondrial fission protein Fis1 regulates yeast mitochondrial fission and is required for ethanol-induced mitochondrial fragmentation and apoptosis. To examine the function of Fis1 in a plant pathogenic fungus, we cloned the MoFIS1 gene, a homolog of Saccharomyces cerevisiae FIS1, from Magnaporthe oryzae and characterized its function by targeted gene deletion and mutant phenotypic analysis. MoFIS1 deletion mutants were unaltered in conidial germination, appressorium formation, and mating tests, but were severely defective in colony growth, conidiation, virulence on rice and barley, growth under nitrogen and glucose deficiency, and growth under osmotic stress. Blast lesions on rice leaves caused by the Delta Mofis1 strain were significantly reduced, were non-proliferating, and were less coalesced as compared to the highly coalesced and proliferating lesions resulting from infection with the wild-type strain. The defects in growth, conidiation, and virulence of the mutant were restored in a complementation strain of Delta Mofis1. A MoFis1-GFP fusion protein co-localized with Mitotracker red in mitochondria. These results show that MoFIS1 encodes a mitochondrial protein that regulates fungal growth, conidiation, and virulence in M. oryzae. (C) 2015 Elsevier GmbH. All rights reserved.
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