Journal
INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
Volume 23, Issue 2, Pages 361-366Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/IGC.0b013e31827cfecb
Keywords
Ovarian cancer; GSK3-beta; Lithium chloride
Categories
Funding
- Barnes-Jewish Hospital Foundation Special Research Program Grant Support for Gynecologic Oncology Research [7519-55]
- Jane Eberle-Newbold Memorial Fund
Ask authors/readers for more resources
Objective: Lithium chloride (LiCl) has been shown to demonstrate anticancer properties at supratherapeutic doses. This study was designed to determine whether LiCl, as a single agent or in combination with cytotoxic agents, reduces ovarian cancer cell growth and metabolic activity at clinically achievable levels. Methods: We studied the effects of LiCl on 2 high-grade serous ovarian cancer cell lines, SKOV3 and OVCA 433, and primary cultures developed from ascitic fluid collected from patients with metastatic high-grade serous ovarian cancer. We assessed proliferation and metabolism using cell cycle analysis, MTT assays, and cellular proliferation and clonogenic potential assays. Results: Treatment with 1 mM LiCl had no effect on the cell cycle distribution or metabolic activity of the SKOV3 and OVCA 433 cell lines. Combination treatment with cisplatin or paclitaxel led to statistically significant decreases in metabolic activity in the OVCA 433 cell line and 50% of cultures investigated. The decreased metabolic activity was not, however, associated with decreased cell growth or clonogenic potential. Conclusions: Combination treatment with LiCl and cytotoxic agents at physiologically achievable drug concentrations reduces ovarian cancer cell metabolism but does not appear to affect cellular proliferation. The potential for combined lithium/cytoxic therapies appears to be limited based on our analysis of both established cell lines and short-term ovarian cancer cultures.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available