Journal
INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
Volume 21, Issue 1, Pages 153-160Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/IGC.0b013e3182022997
Keywords
Complete hydatidiform mole; Malignant transformation; CLIC1; Proteomics
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Funding
- Foundation of Nanjing Medical University [08NMUZ035]
- Key Laboratory for Laboratory Medicine of Jiangsu Province [XK200731]
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Objectives: The purpose of this study was to identify prognostic biomarkers indicating malignant transformation of hydatidiform moles (HMs). Methods: Two-dimensional gel electrophoresis-based proteomic approach was used to compare the protein profiles of complete benign moles (3 samples) with those of malignant-transformed moles (3 samples). Matrix-assisted laser desorption/ionization time of flight mass spectrometry was used to identify differentially expressed proteins. Western blot was used to verify the results of 2-dimensional gel electrophoresis, and immunohistology was used to explore the function of these proteins in gestational trophoblastic disease. Results: Eighteen proteins, deregulated in the malignant-transformed group compared with the benign group (ratio >= 2; P < 0.05), were identified. A bioinformatic analysis indicated that most of these 18 proteins were involved in the processes of cell proliferation and cell survival. Among the 18 proteins, chloride intracellular channel protein 1 (CLIC1) was chosen for further study. Our results showed that the levels of CLIC1 expression in choriocarcinoma tissue were higher than in complete HM tissue (P < 0.01). Chloride intracellular channel protein 1 expression was increased in the tissues of malignant-transformed HMs compared with nontransformed HMs (P < 0.01). Conclusion: Our findings suggest that CLIC1 could be a potential new prognostic biomarker for hydatidiform mole that undergoes malignant transformation.
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