Journal
MICROBIAL PATHOGENESIS
Volume 89, Issue -, Pages 154-160Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2015.10.015
Keywords
Antimicrobial resistance; Hospital settings; Microorganisms; Toxicity
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Funding
- Scientific Research Promotion Fund for the Talents of Jiangsu University [1291330009]
- National Natural Science Foundation of China [31572467]
- National Basic Research Program of China [2012CB114604]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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There are currently great concerns about the level of bacterial contamination in hospitals, as well as resistance to antimicrobial agents. The species and characteristics of microbes in Chinese hospitals are closely related to healthcare safety and the prevention and control of infections. However, data on the exposure of patients to microbes in Chinese hospitals are limited. The present study investigated the genera of microorganisms in Chinese hospitals. We evaluated their characteristics, such as antibiotic susceptibility, tolerance to disinfectants, and toxicity, using silkworms (Bombyx mori) as an animal model. Twenty-six distinct bacterial strains were isolated, and their genera were determined by sequencing their 16S rDNA regions. Twenty-five strains were resistant to one or more antibiotics, and six strains were resistant to multiple antibiotics. The results of minimal inhibitory concentration testing showed that eight strains were resistant to a chlorine-containing disinfectant, and 12 strains were resistant to Povidone iodine. Following the injection of bacterial cultures into the silkworm hemolymph, 15 strains killed all of the silkworms within 5 d. Additionally, bacterial strain 14 killed all of silkworms within 12 h with a median lethal dose of 4 x 10(4) colony-forming units/larva. This study provides useful information for healthcare safety in Chinese hospitals. (C) 2015 Elsevier Ltd. All rights reserved.
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