Journal
INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
Volume 19, Issue 2, Pages 214-222Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1111/IGC.0b013e31819bcb75
Keywords
Cell migration; GPR30; Lipid rafts; Membrane ER; OVCAR-3; uPAR
Categories
Funding
- Swedish Cancer Fund
- Medical Faculty of Lund University
- Crafoords fund
Ask authors/readers for more resources
Epidermal growth factor (EGF) Stimulates proliferation and migration in ovarian cancer cells, and high tumor expression of the EGF system correlates with poor prognosis. Epidermal growth factor upregulates urokinase plasminogen activator receptor (uPAR) on the cell surface via 3 distinct mechanisms: rapid mobilization of uPAR from detergent-resistant domains, increased mRNA, and decreased degradation. G-protein-coupled receptor 30 (GPR30) is a newly identified membrane estrogen receptor (ER). The objective of this study was to explore the effects of 17 beta-cstradiol (E-2) On uPAR expression and cell migration in ovarian cancer cells and further to identify the ER involved. We used 7 ovarian cancer cell lines, cell migration assay, cellular binding of I-125-uPA, cellular degradation of I-121-uPA/PAI-I complex, enzyme-linked immunosorbent assay for uPAR, solid-phase enzyme immunoassay for ER alpha, and quantitative polymerase chain reaction. Estradiol attenuates the stimulatory effect of EGF on cell migration and uPAR expression. Specifically, E-2 reduces the very rapid increase of detergent extractable uPAR. which occurs within minutes of EGF stimulation and probably represents mobilization of uPAR from detergent-resistant domains such as lipid rafts. Estradiol influenced neither the amount of uPAR mRNA nor the rate of uPAR degradation or solubilization. The nuclear ER antagonists ICI 182780 and tamoxifen, which are GPR30 agonists, its well as the specifically constructed GPR30 agonist G I mimicked the effect Of E-2 On uPAR expression and cell migration. OVCAR-3 cells express mRNA for GPR30. Estradiol attenuates EGF-induced mobilization of ligated uPAR from detergent-resistant domains and subsequent migration in ovarian cancer cells. The response to various ER ligands indicates that this effect is mediated via the membrane ER GPR30.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available