Journal
INTERNATIONAL JOURNAL OF GREEN ENERGY
Volume 11, Issue 8, Pages 787-795Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15435075.2013.829779
Keywords
Trichosporon fermentans; Microbial oil; Rice straw hydrolysate; Activated carbon; Biodiesel
Funding
- National Natural Science Foundation of China [31071559, 21072065]
- New Century Excellent Talents in University [NCET-11-0161, NCET-10-0367]
- Open Project Program of the State Key Laboratory of Pulp and Paper Engineering, SCUT [201138]
- National Key Basic Research Program of China [2013CB733500]
- State Key Program of National Natural Science Foundation of China [21336002]
- Fundamental Research Funds for the Central Universities [2014ZZ0048]
- Open Project Program of Provincial Key Laboratory of Green Processing Technology and Product Safety of Natural Products [201207]
- Natural Science Foundation of Guangdong Province [S2012040007546]
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Microbial oil production by Trichosporon fermentans was carried out on the rice straw acid hydrolysate in this work. In order to reduce the detoxification cost, activated carbon was used as absorbent. The effect of peptone concentration, inoculum size, cultural temperature, and initial pH on the lipid fermentation of T. fermentans was evaluated that initial pH showed more significant influence than other three factors. The optimal peptone concentration, inoculum size, cultural temperature, and initial pH for the lipid production were 0.6 g/L, 10%, 25 degrees C, and 7.0, respectively. Under the optimal condition, the highest lipid yield of T. fermentans was 12.1 g/L (corresponding to 31.0 g/L biomass and 39.0% lipid content) after 10 days' fermentation. This work showed that low-cost activated carbon could be utilized for detoxification of rice straw acid hydrolysate, and T. fermentans is a promising strain for microbial oil and thus biodiesel production from lignocellulosic biomass.
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