4.5 Article

Carotenoid content and in vitro bioaccessibility of lycopene from guava (Psidium guajava) and watermelon (Citrullus lanatus) by high-performance liquid chromatography diode array detection

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Publisher

TAYLOR & FRANCIS LTD
DOI: 10.3109/09637480801987195

Keywords

Lycopene; carotenoids; in vitro bioaccessibility; Psidium guajava; Citrullus lanatus

Funding

  1. International Foundation for Science, Stockholm, Sweden
  2. Organisation for the Prohibition of Chemical Weapons (OPCW), THE HAGUE, Netherlands [E/3655-1]
  3. National Research Council [05-36]

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The carotenoid content and in vitro accessibility of the 'Sugar baby' variety of watermelon and the 'Horana red' variety of guava from Sri Lanka was determined using high-performance liquid chromatography. The high-performance liquid chromatography chromatogram showed that the Guava 'Horana red' variety contained almost exclusively lycopene (45.3 +/- 8.0 mu g/g fresh weight (FW)), with a small amount of lutein (2.1 +/- 0.6 mu g/g FW), beta-carotene (2.0 +/- 0.2 mu g/g FW) and beta-cryptoxanthin. As far as carotenoids in the sugar baby variety of watermelon are concerned, it contained lycopene, lutein and beta-carotene of 37.2 +/- 4.0 mu g/g FW, 2.1 +/- 0.6 mu g/g FW and 0.3 +/- 1 mu g/g FW, respectively. The studies showed that guava contains more lycopene (45.3 +/- 8.0 mu g/g FW) than watermelon (37.2 +/- 4.0 mu g/g FW), and that the in vitro accessibility of lycopene in guava (73%) is more than that in watermelon (25.8%). Therefore it can be concluded that guava can be used as a better lycopene source than watermelon.

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