Journal
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
Volume 44, Issue 8, Pages 1602-1608Publisher
WILEY
DOI: 10.1111/j.1365-2621.2008.01901.x
Keywords
Acetes chinensis; alcalase 2; 4L; enzymatic hydrolysis; free radical scavenging activity; molecular weight distribution; response surface methodology
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Funding
- Guangdong Ocean University [0512139]
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P>Response surface methodology (RSM) was used to optimise the hydrolysis parameters of Acetes chinensis by Alcalase 2.4L in order to obtain a hydrolysate with potent radical scavenging activity. The parameters were temperature, pH and enzyme concentration/substrate concentration (E/S) ratio with degree of hydrolysis (DH) being the response. The results showed that the optimum condition was: temperature at 57 degrees C, pH at 8.0, E/S ratio at 2.6AU 100 g(-1) shrimp, hydrolysis time 3 h. The DH was 26.32%, the hydroxyl radical scavenging activity was up to 88.12% and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was 35.61%. The gel column filtration chromatography by a Sephadex G-25 column yielded five fractions. The molecular weight of the most potent free radical scavenging activity fraction ranged from 915 to 207 Da, its IC50 for hydroxyl radical was 0.03 mg mL(-1), and IC50 for DPPH radical was 8.86 mg mL(-1).
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