Journal
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
Volume 44, Issue 12, Pages 2394-2401Publisher
WILEY
DOI: 10.1111/j.1365-2621.2008.01876.x
Keywords
Antioxidant activity; colour; extraction; grape seed; polyphenols; proanthocyanidins; solvent; temperature
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Funding
- Ministry of Science, Education and Sports of the Republic of Croatia [113-1130471-0338]
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P>This study was aimed to investigate the influence of solvent (water and ethanol in concentration: 50%, 70% and 96%) and extraction temperature (25-80) degrees C on polyphenols extraction of grape seed (Vitis vinifera L. cv. Frankovka) cultivated in Eastern Croatia. The best results were reached using 50% ethanol at 80 degrees C. The most abundant individual polyphenolic compound was catechin which makes the average of 45.11% of the total phenolic content followed by epicatechin (34.45%), procyanidin B-2 (12.90%), gallic acid (5.34%), gallocatechin (1.58%), epicatechin gallate (1.01%). The antioxidant activity of extracts was determined by DPPH-method and it was highly dependent on extraction solvent and temperature. The total extractable proanthocyanidins content of extract had the highest positive correlation (r = 0.994) with antioxidant activity, which indicated that these compounds might be the most important antioxidant in examined grape seed extracts. Relationship between phenolic content and colour of extracts was observed.
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