Journal
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
Volume 14, Issue 3, Pages 514-522Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10942910903256964
Keywords
UPLC; Spectrophotometry; Wine; Flavanols; Flavonols; Total wine polyphenols; Anthocyanins
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Funding
- Polish Ministry of Sciences [N N305 267834]
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Ultra performance liquid chromatography-photodiode array detection (UPLC-PDA) was used to analyze five biologically active polyphenolic compounds (catechin, epicatechin, rutin, trans-resveratrol, and quercetin) in different types of wine available in the Polish market. The results showed that the major polyphenols in chosen wines were catechin and epicatechin, the minor polyphenol was trans-resveratrol. The use of UPLC system allowed a shorter run time of up to six times in comparison with the conventional HPLC system. Total polyphenols were analyzed according to the Folin-Ciocalteu method using gallic acid as a standard and the results were presented as gallic acid equivalents. The wines were also analyzed for anthocyanins content using malvidin-3,5-diglucoside as a reference material in spectrophotometric analysis.
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