Journal
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
Volume 175, Issue -, Pages 30-35Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijfoodmicro.2014.01.015
Keywords
Alicyclobacillus acidoterrestris; Immunomagnetic separation; Real-time PCR; Detection; Fruit products
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Funding
- National Science Technology [2012BAK17B06, 2012BAD31801]
- National Natural Science Foundation of China [31171721,31371814]
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Alicyclobacillus acidoterrestris is the most important spoilage species within the Alicyclobacillus genus and has become a major issue in the pasteurized fruit juice industry. The aim of this study was to develop a method combining immunomagnetic separation (IMS) with real-time PCR system (IMS-PCR) for rapid and specific detection of A. acidoterrestris in fruit products. A real-time PCR with the TaqMan system was designed to target the 16S rDNA genes with specific primer and probe set. The specificity of the assay was confirmed using 9 A. acidoterrestris strains and 21 non-A. acidoterrestris strains. The results indicated that no combination of the designed primers and probe was found in any Alicyclobacillus genus except A. acidoterrestris. The detection limit of the established IMS-PCR was less than 10 CFU/mL and the testing process was accomplished in 2-3 h. For the three types of samples (sterile water, apple juice and kiwi juice), the correlation coefficient of standard curves was greater than 0.991, and the calculated PCR efficiencies were from 108% to 109%. As compared with the standard culture method performed concurrently on the same set of samples, the sensitivity, specificity and accuracy of IMS-PCR for 196 naturally contaminated fruit products were 90.0%, 983% and 97.5%, respectively. The results exhibited that the proposed IMS-PCR method was effective for the rapid detection of A. acidoterrestris in fruit products. (C) 2014 Published by Elsevier B.V.
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