Journal
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
Volume 164, Issue 1, Pages 76-80Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijfoodmicro.2013.03.023
Keywords
Paratuberculosis; Phage-PCR assay; Detection; Milk
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Funding
- EU
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Conventional culture and a rapid phage-PCR method were used to detect Mycobacterium avium subsp. paratuberculosis (MAP) in bulk tank milk (BTM) samples. Only two of 225 samples (0.9%) were found to contain MAP by culture whereas 50 (22%) MAP-positive samples were identified using the phage-PCR assay, including both samples that were MAP-culture positive. Results using the phage-based method for independently tested duplicate samples indicated that the assay is very reproducible (r(2) = 0.897), especially when low levels of mycobacteria are present. A relationship was established between plaque number and the presence of MAP in a sample. A cut-off value was determined allowing identification of MAP-positive samples based on plaque number alone (90% sensitivity, 99% specificity; area under the curve = 0.976). These results indicate that the assay is a robust method for screening BTM, providing results within 24 h. (C) 2013 Elsevier B.V. All rights reserved.
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