Journal
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
Volume 54, Issue 5, Pages 887-896Publisher
UNIV BASQUE COUNTRY UPV-EHU PRESS
DOI: 10.1387/ijdb.092948ow
Keywords
invasion; collagen; stroma; 3D matrices; ecosystem
Categories
Funding
- Fund for Scientific Research-Flanders (Brussels, Belgium) [FWOAL455]
- INSERM
- ARC
- Flemish community and France [1.2007.03, T2009.14]
- EACR
- Fund for Scientific Research-Flanders
- National Human Genome Research Institute
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Tumor invasion is the outcome of a complex interplay between cancer cells and the stromal environment. Considering the contribution of the stromal environment, we developed a membrane-free single-cell and spheroid based complementary model to study cancer invasion through native collagen type-I matrices. Cell morphology is preserved during the assays allowing real time monitoring of invasion-induced changes in cell structure and F-actin organization. Combining these models with computerized quantification permits the calculation of highly reproducible and operator-independent data. These assays are versatile in the use of fluorescent probes and have a flexible kinetic endpoint. Once the optimal experimental conditions are empirically determined, the collagen type-I invasion assays can be used for preclinical validation of small-molecule inhibitors targeting invasion. Initiation and monitoring of the single-cell and spheroid invasion model can be achieved in 8 h (over 3 days) and in 14 h (over 8 days) respectively.
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