Journal
MEMORIAS DO INSTITUTO OSWALDO CRUZ
Volume 110, Issue 2, Pages 272-274Publisher
FUNDACO OSWALDO CRUZ
DOI: 10.1590/0074-02760140371
Keywords
Strongyloides stercoralis; molecular diagnosis; conventional PCR; real-time PCR
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Funding
- FAPESP [2010/51110-2]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [10/51110-2] Funding Source: FAPESP
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This study aimed to evaluate the use of conventional polymerase chain reaction (cPCR) and real-time quantitative PCR (qPCR) in the diagnosis of human strongyloidiasis from stool samples in tropical areas. Stool samples were collected from individuals and were determined to be positive for Strongyloides stercoralis (group I), negative for S. stercoralis (group II) and positive for other enteroparasite species (group III). DNA specific to S. stercoralis was found in 76.7% of group I samples by cPCR and in 90% of group I samples by qPCR. The results show that molecular methods can be used as alternative tools for detecting S. stercoralis in human stool samples in tropical areas.
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