Journal
INTERNATIONAL JOURNAL OF CARDIOLOGY
Volume 175, Issue 3, Pages 545-553Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.ijcard.2014.06.034
Keywords
Stem cell; Tissue engineering; Myocardial infarction; Adipose-derived regenerative cells; Magnetic nanoparticle
Categories
Funding
- Japan Science and Technology Agency [26-J-Jd08]
- Ministry of Education, Culture, Sports, Science and Technology of Japan [20249045]
- Banyu Life Science Foundation International
- Kanae Foundation
- Kowa Life Science Foundation
- Grants-in-Aid for Scientific Research [20249045, 26293184, 25860595] Funding Source: KAKEN
Ask authors/readers for more resources
Background: Adipose-derived regenerative cells (ADRCs) are a promising source of autologous stem cells for regeneration and repair of damaged tissue. Herein, we investigated the therapeutic potential of ADRC sheets created by a magnetite tissue engineering technology (Mag-TE) for myocardial infarction. Methods and results: Adipose tissue was obtained from wild-type (WT) mice and ADRCs were isolated. ADRCs incubated with magnetic nanoparticle-containing liposomes (MCLs) were cultured. MCL-labeled ADRCs were mixed with a diluted extracellular matrix (ECM) precursor, and a magnet was placed on the reverse side. Magnetized ADRCs formed multilayered cell sheets after a 24-h incubation. WT mice were subjected to myocardial infarction by permanent ligation of the left anterior descending artery. We then transplanted the constructed ADRC sheet or a cell-free collagen gel sheet, as a control, onto the infarcted myocardium using an Alnico magnet before skin closure. Cardiac parameters were measured by echocardiogram, and angiogenesis was determined by tissue capillary density. ADRC sheet-treated mice showed significant improvements in systolic function, infarct wall thinning, and fibrotic length after myocardial infarction. ADRC sheet implantation also promoted angiogenesis in both the infarct area and the border zone in WT mice after myocardial infarction. The angiogenic effects of ADRC sheets were attributed to an increased expression of VEGF and bFGF mRNA in ischemic hearts. Conclusions: ADRC sheets created by this Mag-TE method protect the heart against pathological cardiac remodeling. Our ADRC sheets have the potential to be a novel regenerative strategy for ischemic heart disease. (C) 2014 Elsevier Ireland. Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available