Journal
INTERNATIONAL JOURNAL OF CARDIOLOGY
Volume 167, Issue 5, Pages 2039-2046Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.ijcard.2012.05.056
Keywords
Adiponectin; Circulating angiogenic cells; Stromal-derived factor 1; Migration; Exercise training
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Funding
- Deutsche Forschungsgemeinschaft (DFG) [LI946/3-1]
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Aims: Adiponectin (adipo) and exercise training (ET) contribute to the maintenance of a normal vascular tone by influencing vascular NO bioavailability and concentration and function of circulating angiogenic cells (CAC). The molecular mechanisms are only partially understood. Aim of the present study was to elucidate the effects of adipo on CAC migration and the underlying signaling pathways. Furthermore, the impact of ET on adiponectin-mediated CAC migration was investigated. Methods and results: CACs were isolated from peripheral blood and exposed to different adipo concentrations. Adipo (5 mu g/ml) enhanced the ability of CACs to migrate following an SDF-1 gradient by 345%. This was associated with a significant increase in CXCR4 expression on the surface of CACs as compared to control (10.1 +/- 1.5 vs. 33.2 +/- 4.5% CXCR4 positive cells, p<0.05). Adiponectin-induced CAC migration and CXCR4-upregulation were mediated through adipo-receptor 1 (AdipoR1) and blocked by an inhibitor of PI3-kinase, p38MAP kinase and NF kappa b. Adipo-stimulated migration of CACs, CXCR4 expression and p38MAPK-activation is impaired in patients with coronary artery disease (CAD). ET over 4 weeks partially corrects adiponectin-stimulated CAC migration and CXCR4 expression in patients with CAD (n=10). No change was observed in the control group (n=10). Conclusion: Adipo improves the migratory capacity of CACs in response to SDF1, partially through an upregulation of CXCR4. This is mediated through a pathway that involves binding of adipo to the AdipoR1 and subsequent PI3kinase/p38MAPK/NF kappa b activation. In addition ET corrects the adiponectin responsiveness of CACs, and thereby might promote endogenous repair of damaged endothelium. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
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