Journal
INTERNATIONAL JOURNAL OF CANCER
Volume 126, Issue 10, Pages 2437-2447Publisher
WILEY
DOI: 10.1002/ijc.24953
Keywords
circulating tumor cells; chemotherapy; drug resistance
Categories
Funding
- Associazione ricerche Geriatriche ed Oncologiche (Ar.Ger.On.)
- Fondazione per la Ricerca Oncologica (FORO)
- Italian Ministry for University and Research
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The prognostic value associated with the detection of circulating tumor cells (CTCs) in metastatic breast cancer by the CellSearch (TM) technology raise additional issues regarding the biological value of this information. We postulated that a drug-resistance profile of CTCs may predict response to chemotherapy in cancer patients and therefore could be used for patient selection. One hundred 5 patients with diagnosis of carcinoma were enrolled in a prospective trial. CTCs were isolated from peripheral blood, and positive samples were evaluated for the expression of a panel of genes involved in anticancer drugs resistance. The drug-resistance profile was correlated with disease-free survival (DFS; patients in adjuvant setting) and time to progression (TIP; metastatic patients) in a 24-months follow-up. Objective response correlation was a secondary end point. Fifty-one percent of patients were found positive for CTCs while all blood samples from healthy donors were negative. The drug-resistance profile correlates with DFS and TTP (p < 0.001 in both). Sensitivity of the test: able to predict treatment response in 98% of patients. Specificity of the test: 100%; no sample from healthy subject was positive for the presence of CTCs. Positive and negative predictive values were found to be 96.5 and 100%, respectively. We identified a drug-resistance profile of CTCs, which is predictive of response to chemotherapy, independent of tumor type and stage of disease. This approach may represent a first step toward the individualization of chemotherapy in cancer patients.
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