Journal
INTERNATIONAL JOURNAL OF CANCER
Volume 126, Issue 4, Pages 1029-1035Publisher
WILEY
DOI: 10.1002/ijc.24823
Keywords
apoptosis; glioblastoma; Bcl-2; Mcl-1; MiR-153
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Funding
- National Natural Science Foundation of China [30672463]
- National Basic Research Program of China (973-Program) [2006CB50390]
- Chinese Academy of Sciences [KSCX2-YW-R-085]
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MicroRNA-153 (miR-153) is a brain-specific miRNA that is expressed at a significantly lower level in glioblastoma (GBM) relative to non-neoplastic brain tissue. Although the expression pattern of miR-153 has been extensively established, its target genes and cellular mechanism remain undefined. To investigate into the potential function of miR-153 in glioblastmas, we transfected a GBM cell line DBTRG-05MG with synthetic miR-153 oligos and observed decreased cell proliferation and increased apoptosis. Bioinformatics analysis revealed that anti-apoptosis family member B-cell lymphoma 2 (Bcl-2) and myeloid cell leukemia sequence 1 (Mcl-1) are potential targets of miR-153. Indeed, Western blot analysis indicated that miR-153 downregulated both Bcl-2 and Mcl-1 at the protein levels. Single strand miR-153 inhibitor, which forms complementary base-pair with endogenous miR-153, efficiently blocked the apoptosis and target protein degradation induced by overexpression of miR-153. By luciferase reporter assays, we further showed that miR-153 inhibited Bcl-2 and Mcl-1 expressions by directly targeting the 3'UTR regions of their respective mRNAs.
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