Journal
INTERNATIONAL JOURNAL OF CANCER
Volume 124, Issue 6, Pages 1440-1448Publisher
WILEY
DOI: 10.1002/ijc.24067
Keywords
interleukin-13 receptor; squamous cell carcinoma; oral cancer; synergistic effect; gene transfer; retrovirus; paclitaxel; orthotopic animal model
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Interleukin-13 receptor-targeted cytotoxin (IL13-PE38) is highly cytotoxic to certain types of human cancers expressing abundant levels of IL-13R alpha 2 chain. Although IL13-PE38 is being tested in a Phase III clinical trial in brain tumors, the activity of IL13-PE38 alone or when combined with taxane, a chemotherapeutic drug for oral squamous cell carcinoma (OSCC), has not been investigated. Here, we show that approximately 40% of OSCCs (n = 150) in a tissue array are strongly positive for IL-13R alpha 2, whereas normal oral mucosa (n = 10) expresses very low or undetectable levels evaluated by immunohistochemistry. IL13-PE38 was highly cytotoxic to OSCC cell lines, but not cytotoxic to normal oral fibroblasts. IL13-PE38 mediated a synergistic antitumor effect with paclitaxel in OSC-19 in vitro and in vivo in the orthotopic OSCC tongue tumor model. Real-time tumor growth was monitored by optical imaging using a Xenogen-IVIS imaging system. Treated animals showed significant (p < 0.05) improvement in survival, which correlated with in vivo imaging of tumor response without evidence of visible toxicity. Gene transfer of IL-13Ra2 in oral cancer cells increased sensitivity of OSCC cell line to IL13-PE38 in vitro. Retro-virus-mediated gene-transfer of IL-13R alpha 2 in HSC-3 into tongue tumors in vivo dramatically enhanced the antitumor activity of IL13-PE38, providing complete elimination of established tumors and prolonging survival of these animals. These results indicate that IL13-PE38 in combination with paclitaxel acting via different mechanisms may be a potential treatment option for IL-13R alpha 2 expressing OSCC or for the treatment of nom-IL-13R alpha 2 expressing OSCC combined with gene transfer of IL-13R alpha 2. Published 2008 Wiley-Liss, Inc.
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