Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
Volume 10, Issue 3, Pages 338-349Publisher
IVYSPRING INT PUBL
DOI: 10.7150/ijbs.7972
Keywords
endurance exercise; Fndc5; irisin; myokine; Ppargc1a
Categories
Funding
- Leibniz Association [SAW-2013-FBN-3]
- Deutsche Forschungsgemeinschaft [DFG H0 2003/6-1]
Ask authors/readers for more resources
Recent findings regarding the response of fibronectin type III domain-containing protein 5 (Fndc5) and irisin to exercise are partly controversial. While the 25 kDa form of Fndc5 can be observed in muscle and serum of different species, the similar to 12 kDa irisin band was not detectable up to now. The present study aimed to clarify whether irisin exists in its theoretical size of similar to 12 kDa in mice and if it is affected by exercise. Male mice were randomly assigned to a sedentary control group (CO), a group with free access to running wheels (RW), and a treadmill group (TM). Blood and leg muscles were collected to investigate the regulatory cascade including peroxisome proliferator-activated receptor gamma co-activator 1-alpha (Ppargc1a) and Fndc5. In western blot analysis, antibodies were used capable of differentiation between full-length Fndc5 and irisin. This enabled us to demonstrate that irisin exists in muscle and serum of mice independent of exercise and that it is increased immediately after acute exercise. Different transcripts of Ppargc1a mRNA, but not Fndc5 mRNA, were up-regulated in the TM group. Furthermore, neither Fndc5 (25 kDa) nor Ppargc1a protein was elevated in muscle tissue. The Ppargc1a-Fndc5/irisin pathway did not clearly respond to mild exercise in the RW group. Our results provide evidence for the existence of irisin and for its immediate response to acute exercise in mice.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available