Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
Volume 10, Issue 9, Pages 1064-1071Publisher
IVYSPRING INT PUBL
DOI: 10.7150/ijbs.8415
Keywords
FGFR1; Endotoxemia; LPS; EPCs; Osteoblast; SDF-1 alpha
Categories
Funding
- Special Funds for Major State Basic Research Program of China (973 program) [2014CB942904]
- National Natural Science Foundation of China [81170809, 81471092]
- Fundation of army [CWS11J322]
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Endothelial progenitor cells (EPCs) contribute to neovascularization and vascular repair, and may exert a beneficial effect on the clinical outcome of sepsis. Osteoblasts act as a component of niche in bone marrow, which provides a nest for stem/progenitor cells and are involved in the formation and maintenance of stem/progenitor cells. Fibroblast growth factor receptor 1 (FGFR1) can regulate osteoblast activity and influence bone mass. So we explored the role of FGFR1 in EPC mobilization. Male mice with osteoblast-specific knockout of Fgfrl (Fgfr1(fl/fl);OC-Cre) and its wild-type littermates (Fgfr1(fl/fl)) were used in this study. Mice intraperitoneally injected with lipopolysaccharide (LPS) were used to measure the number of circulating EPCs in peripheral blood and serum stromal cell-derived factor 1a (SDF-1a). The circulating EPC number and the serum level of SDF-1a were significantly higher in Fgfr1(fl/fl); OC-Cre mice than those in Fgfr1(fl/fl) mice after LPS injection. In cell culture system, SDF-1a level was also significantly higher in Fgfr1(fl/fl); OC-Cre osteoblasts compared with that in Fgfr1(fl/fl) osteoblasts after LPS treatment. TRAP staining showed that there was no significant difference between the osteoclast activity of septic Fgfr1(fl/fl) and Fgfr1(fl/fl); OC-Cre mice. This study suggests that targeted deletion of Fgfr1 in osteoblasts enhances mobilization of EPCs into peripheral blood through up-regulating SDF-1a secretion from osteoblasts.
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