Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 120, Issue -, Pages 1544-1550Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2018.09.123
Keywords
Codonopsis pilosula; Polysaccharides; Anti-tumor activity
Funding
- Projects of Science and Technology Agency of Gansu [1504FKCA010]
- Science and Technology Agency of Lanzhou [2016-2-8, 2017-4-119]
- major projects of Science and Technology Department of Gansu Province [17ZD2FA009]
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Two water-soluble polysaccharides named CPP1a and CPP1c were isolated from C. pilosula Nannf. var. modesta L.T.Shen by hot-water extraction and purified by graded alcohol precipitation and DEAE-52 cellulose column. The structure of CPP1c with higher yield has been characterized while its antitumor activities has not been elucidated. In this study, we firstly analyzed the chemical structure of CPP1a. The results of instrumental analysis combined with chemical analysis showed that CPP1a was composed of -> 1)-beta-L-Rhap-(4 ->, -> 1)-beta-Arap-(5 ->, -> 1)-beta-D-GalpA-(4 ->, -> 1)-beta-D-Galp-(6 ->, terminal-beta-D-Glcp in a molar ratio of 1:12:1:10:3 and its relative and absolute molecular weight were 1.01 x 10(5) Da and 1.03 x 10(5) Da respectively. Further, the cytotoxicity assay indicated that CPP1a and CPP1c were more sensitive to HepG2 cells than cervical carcinoma Hela cells and gastric carcinoma MKN45 cells. Both of CPP1a and CPP1c could influence cell morphology, inhibit the migration and induce apoptosis by affecting the G2/M phase of HepG2 cells. Preliminary mechanism studies confirmed that CPP1a and CPP1c could induce apoptosis through up-regulating the ratio of Bax/Bcl-2 and activating caspase-3. According to previous research, we might speculate that the reason for the stronger cytotoxicity and pro-apoptotic effect of CPP1c than that of CPP1a can be attributed to its high uronic acid content. (C) 2018 Elsevier B.V. All rights reserved.
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