Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 125, Issue -, Pages 1184-1191Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.09.113
Keywords
Fenugreek gum; Sulfation; Box-Behnken design
Funding
- National Science Foundation of China [21204073, 51463022]
- Science Fund for Distinguished Young Scholars of Gansu Province [17JR5RA067]
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Sulfated derivatives of the galactomannan family have multiple biological activities. In the present study, sulfated galactomannan from fenugreek gum (SFG) were prepared by chlorosulfuric acid/pyridine (CSA/Py) method. In order to acquire the derivatives with the highest degree of substitution (DS), the experiment optimum conditions of the sulfation were determined by Box-Behnken design (BBD). The quadratic regression model analysis confirmed that reaction time was the most significant parameter for the impact of DS. Under the selected conditions, maximum DS was obtained as 0.490. Results of FT-IR and X-ray photoelectron spectroscopy (XPS) indicated the present of -SO3- group. In C-13 NMR spectroscopy, the original C-6 peaks did not disappear completely and the newly peaks appeared at delta 63.2 and 64.0 illustrating the incomplete substitution were predominantly on C-6 position. After sulfation, size exclusion chromatography combined with multi-angle laser light scattering (SEC-MALLS) detected all the weight average molecular mass (Mw) of sulfated derivatives decrease rapidly. The introduction of the electrostatic interaction of negatively charged -SO3- groups and the decrease in Mw could have a significant effect on its biological activity. (C) 2018 Elsevier B.V. All rights reserved.
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