Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 120, Issue -, Pages 10-18Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.08.063
Keywords
Unfolded protein; Coacervation; Aggregation
Funding
- JSPS KAKENHI [17J03290, 18H02383]
Ask authors/readers for more resources
Coacervates are self-assemblies formed by oppositely charged macromolecules in aqueous solution. Although coacervates usually take a homogeneous spherical shape with flowability, they have the potential to adopt unexpected macroscopic structures. In this study, we investigated the influence of the interaction mode and morphology on unfolded proteins constituting coacervates and coaggregates using ovalbumin (OVA) and lysozyme (LYZ) as the model systems. The unfolded proteins were prepared via heating at 80 degrees C and then incubated at ambient temperature. OVA and LYZ formed complexes at pH values between their respective isoelectric points in both their native and unfolded states. The unfolded proteins were more prone to form complexes than the native proteins due to hydrophobic interactions rather than electrostatic attraction. Interestingly, native OVA and LYZ formed liquid-like coacervates with spherical shapes, whereas unfolded OVA and LYZ formed solid-like coaggregates with amorphous structures. Understanding the difference between coacervates and coaggregates will provide fundamental information regarding differences between the amorphous aggregation and liquid liquid phase separation of proteins. (C) 2018 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available