Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 120, Issue -, Pages 152-158Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.08.015
Keywords
Hydroxybutyl chitosan; Thermosensitive hydrogels; Polydopamine; Cell sheet transplantation
Funding
- National Natural Science Foundation of China [81671828, U1706212]
- Applied Basic Research Plan of Qingdao [16-5-1-70-jch]
- Taishan Scholar Program, China
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The aim of this study was to develop an effective cell sheet translocation method using a cell adhesive and temperature-responsive hydroxybutyl chitosan hydrogel (HBC). The polydopamine (PD)-coated HBC hydrogels were prepared by the dopamine self-polymerization on the surface of HBC hydrogel with different coating time, termed as P30, P60 and P120, respectively. Gelling property of HBC was not affected by PD coating. The PD-coated HBC hydrogels promoted the attachment and proliferation of mouse fibroblast cells (L929) and human umbilical vein endothelial cells (HUVECs), and allowed formation of monolayer cell sheet. In vitro translocation of HUVECs sheet could be obtained successively through phase transition of PD coated HBC hydrogel from gel to sol, and the cells sheet transferred from P30 hydrogel to a round cell coverglass maintained relatively complete monolayer and normal cell morphology. The results showed that P30 hydrogel has the potential to be used for cell transplantation therapy. (C) 2018 Published by Elsevier B.V.
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