Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 64, Issue -, Pages 69-75Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2013.11.030
Keywords
Lycium ruthenicum; Polysaccharide; Structure; Pectin; Immunomodulating activity
Funding
- National Natural Science Foundation of China [31072065, 31372347]
- Twelfth Five Years National Science and Technology Support Plan Project [2011BAD26B02-3]
- National High Technology Research and Development Program of China (863 Program) [2012AA021501]
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An immunologically active pectin, named LRGP5, was firstly isolated from the fruits of Lycium ruthenicum Murr. It contained rhamnose, arabinose, xylose, galactose and galacturonic acid in the molar ratio of 1.0:2.2:0.5:1.2:4.7. Its molecular weight was estimated to be approximately 1.37 x 10(5) Da by high performance gel permeation chromatography. The structure was elucidated using methylation analysis, partial acid hydrolysis, NMR and ESI-MS analysis. Results showed that LRGP5 consisted of a (1 -> 4)-linked galacturonic acid backbone occasionally interrupted by (1 -> 2)-linked rhamnose. The side chains were attached to position 4 of the rhamnosyl units, including (1 -> 3)-linked arabinose, (1 -> 3)-linked galactose, (1 -> 3,6)-linked galactose, (1 -> 4)-linked galacturonic acid, (1 -> 2)-linked rhamnose and (1 -> 2,4)-linked rhamnose, and the termini were arabinose and rhamnose. Immunological assay results demonstrated that LRGP5 could significantly promote macrophage proliferation and enhance the secretion of nitrogen monoxide in vitro. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.
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