Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 64, Issue -, Pages 252-256Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2013.12.011
Keywords
Pullulan; Response surface methodology; Agri-industrial waste
Funding
- Council of Scientific and Industrial Research (CSIR), Government of India
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Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components. A second order polynomial model was obtained using central composite design to understand the effects of interactions among these substrates on pullulan biosynthesis. Results indicated that, lower concentrations of CSL and DOJSC and higher concentrations of jaggery favoured pullulan production. The optimal nutrient composition (18% jaggery, 3% DOJSC and 0.97% CSL) as suggested by the model resulted in production of 66.25 g/L pullulan with a productivity of 0.92 g/L h. Analysis of raw material cost component for pullulan production suggested that sole utilization of agri-residues may lead to development of cost effective process for pullulan production. (C) 2013 Elsevier B.V. All rights reserved.
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