Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 69, Issue -, Pages 561-566Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2014.05.045
Keywords
Polysaccharide; Pleurotus ostreatus; Immunomodulation
Funding
- Youth Foundation of Science and Technology Department of Jilin Province [201201143]
- National Natural Science Foundation of China [31100566]
- Scientific Foundation of Education Department of Jilin Province [2010-131, 2013-162, 2013-169, 2013-352]
- [20140204039YY]
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Pleurotus ostreatus is popularly consumed as traditional medicine and health food for enhancing immune function in China. Polysaccharides from mushroom have been demonstrated to possess a wide range of health beneficial properties. This study was carried out to elucidate the immunomodulating effects and molecular mechanism involved in the in vivo and in vitro anti-tumor activities of alkali-extracted polysaccharide (WPOP-N1) from the fruiting bodies of P. ostreatus. The results showed that WPOP-N1 significantly inhibited the tumor growth of Sarcoma 180 tumor-bearing mice, and markedly increased the secretion level of TNF-alpha in serum. In addition, WPOP-N1 enhanced the phagocytic capability of peritoneal macrophages in vitro. Furthermore, the secretion of TNF-alpha and NO and the amount of TNF-alpha and iNOS transcript were increased significantly when the peritoneal macrophages were exposed to WPOP-N1. Meanwhile, Western blot analysis revealed that the stimulation of peritoneal macrophages by WPOP-N1 induced the phosphorylation of p65 and a marked decrease of I kappa B expression. These results suggest that WPOP-N1 could activate macrophages through NF-kappa B signaling pathway, and the anti-tumor effects of WPOP-N1 can be achieved by its immunostimulating property. (C) 2014 Elsevier B.V. All rights reserved.
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