Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 70, Issue -, Pages 86-91Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2014.06.032
Keywords
Glucuronomannan; Structural analysis; Rheology; NMR; Cyathea medullaris
Funding
- New Zealand Ministry of Business, Innovation and the Environment
- Massey University
- Ministry of Science and Innovation, New Zealand
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A shear-thickening water-soluble polysaccharide was purified from mucilage extracted from the fronds of the New Zealand black tree fern (Cyathea medullaris or 'mamaku' in Maori) and its structure characterised. Constituent sugar analysis by three complementary methods, combined with linkage analysis (of carboxyl reduced samples) and H-1 and C-13 nuclear magnetic resonance spectroscopy (NMR) revealed a glucuronomannan comprising a backbone of 4-linked methylesterified glucopyranosyl uronic acid and 2-linked mannopyranosyl residues, branched at 0-3 of 45% and at both 0-3 and 0-4 of 53% of the mannopyranosyl residues with side chains likely comprising terminal xylopyranosyl, terminal galactopyranosyl, non-methylesterified terminal glucopyranosyl uronic acid and 3-linked glucopyranosyl uronic acid residues. The weight-average molecular weight of the purified polysaccharide was similar to 1.9 x 10(6) Da as determined by size-exclusion chromatography coupled with multi-angle laser light scattering (SEC-MALLS). The distinctive rheological properties of this polysaccharide are discussed in relation to its structure. (C) 2014 Elsevier B.V. All rights reserved.
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