Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 64, Issue -, Pages 302-305Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2013.11.037
Keywords
Chitooligomers; Size; Antibacterial activity
Funding
- National High Technology Research and Development Program (863Program) of China [2011AA09070405]
- commonweal item of State Oceanic Administration People's Republic of China [201305016]
- National Key Technologies RD Program [2011BAE06B04]
- Action Plan of CAS [2011A090100035]
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The antimicrobial activity of chitooligomers (COSs) has attracted considerable interest but there are few reports on antibacterial activity of each individual COS. This study focuses on investigating the size and pH effects of COS on its antibacterial activity against Staphylococcus aureus. Minimum inhibitory concentrations (MICs) of five single COSs and five COS fractions with narrow degrees of polymerization (DP) were determined at different pH mediums. The results revealed that the antibacterial activity of COS required structural essential with a DP of at least 5 and the inhibitory effect increased with increasing DP. Lower pH value could enhance the antibacterial activity of COS. The COS with DP > 12 showed a MIC value of 62.5 mg/mL at pH 6.0, while the MIC value increased to 500 and 1000 mg/mL at pH 6.5 and 7.0, respectively. The cell membrane integrity assay and SEM suggested that the COS with high DP could cause bacteria clustering and further lysis of cell. (C) 2013 Elsevier B.V. All rights reserved.
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