Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 63, Issue -, Pages 8-14Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2013.10.027
Keywords
Chitinase; Chitosanase; Squid pen; Bacillus cereus; Chitinase inhibitor; Boron
Funding
- National Science Council, Taiwan [NSC 102-2313-B-032-001-MY3, NSC 102-2621-M-032-005, NSC 102-2811-B-032-001]
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A chitinase and a chitosanase were induced from a squid pen powder (SPP)-containing medium of Bacillus cereus TKU030 and purified by precipitation with ammonium sulphate and combined column chromatography. The purified chitinase and chitosanase exhibited optimum activity at 60 degrees C, pH 5-6 and 40 degrees C, pH 4, respectively. The chitinase and chitosanase were stable at 25-60 degrees C, pH 4-7 and 25-50 degrees C, pH 3-7, respectively. The chitinase and chitosanase showed the highest activity toward beta-chitin and 60% DD chitosan, respectively. The chitinase was significantly inhibited by Mn2+ and EDTA but activated by Cu2+, Fe2+ and Ca2+. The chitosanase was significantly inhibited by Cu2+, Fe2*, Zn2+, Mn2+ and EDTA. The chitinase showed high stability in the presence of various surfactants, such as SDS, Tween 20, Tween 40 and Triton X-100. In contrast, these surfactants were inhibitors of the chitosanase. The chitinase and chitosanase were also inhibited by TKUPSP017, a small synthetic boron-containing molecule with a BF3K side-chain. However, TKUPSP017 enhanced the growth of B. cereus TKU030 in SPP-containing medium. (C) 2013 Elsevier B.V. All rights reserved.
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