Immune-enhancing activity of polysaccharides from Cyrtomium macrophyllum

The goal of the present study was to investigate the immune-enhancing activity of polysaccharides from Cyrtomium macrophyllum (CMP). Two experiments were carried out. In immunosuppression experiment, the immune-enhancing effect of CMP in immunosuppressive mice was performed. The results showed that CMP at high and medium doses was able to overcome the CY-induced immunosuppression, significantly increases the thymus and spleen indices, enhances lymphocyte proliferation activity and macrophage function, improves immunoglobulin and cytokines levels compared with negative control group. In macrophage immunomodulatory experiment, the immune-enhancing effect of CMP in RAW264.7 cells was measured. The results showed that CMP induced the elevation of NO production, TNF-alpha secretion and iNOS protein of RAW264.7 cells. CMP can also strongly increase NF-kappa B levels in nuclear, which is an important transcription factor that can modulate expressions of iNOS, NO and TNF-alpha. Therefore, the CMP could be an effective immunomodulatory agent. (C) 2014 Elsevier B.V. All rights reserved.