Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 55, Issue -, Pages 83-87Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2012.12.017
Keywords
Polysaccharide; Zizyphus jujuba cv. Junzao; Purification; Structure
Funding
- Special Fund for Agro-scienticfic Research in the Public Interest [201303073]
- National Natural Science Foundation of China [31101361]
- Research Fund of National 12th Five-Year Plan of China [2011AA100806-3]
- Fundamental Research Funds for the Central Universities [JUSRP211A30]
- Major in Jiangsu province science and technology supporting and independent innovation project [20576049]
- Nanjing City SME Technology Innovation Fund Project [2012 Chuangji 056]
- Suzhou Key Technology R&D Program of the Administration of Science and Technology [SN201037]
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A water-soluble polysaccharide (ZP2a) was obtained by extraction with hot water followed by successive chromatographic purification using anion exchange and Sepharose CL-6B columns. A symmetrical narrow peak was obtained on high-performance gel permeation chromatography with an average molecular weight of 120,645 Da, which was named ZP2a. ZP2a is composed of rhamnose, arabinose, glucose and galactose in the molar ratio 1.3:1.7:0.3:1. Based on gas chromatographic, infrared spectral, methylation and NMR analyses, ZP2a was structurally characterized as 1,4-D-GalpA residues interspersed with 1,2-L-Rhap and 1,2,4-L-Rhap residues. The branches were composed of 1,5-L-Araf, 1,3,5-L-Araf, 1,3-L-Araf, 1,6-D-Galp, 1,4,6-D-Galp and 1,4-D-Glcp. The branches were attached to the backbone at the 0-4 position of Rhap residues. (C) 2012 Elsevier B.V. All rights reserved.
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