Selenylation modification can enhance antioxidant activity of Potentilla anserina L. polysaccharide

Potentilla anserina L polysaccharide had been selenizingly modified by nitric acid-selenious acid method with microwave-assistant. The optimal reaction parameters on selenylation modification were time 116 min, temperature 63 degrees C, microwave power 190W and the actual value of selenium content was 2690.1 +/- 7.2 mu g/g. The scavenging ability of DPPH, OH and superoxide radicals, the metal chelating ability and reducing power in vitro were compared by antioxidant assay taking the non-modified PAP as a control. The results showed that selenylation modification could significantly enhance the antioxidant activity of SePAP and SePAP could be used as a potential antioxidant. (C) 2013 Elsevier B.V. All rights reserved.