Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 59, Issue -, Pages 90-95Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2013.04.020
Keywords
Wampee seeds; Acidic polysaccharide; Monosaccharide composition; Antioxidant ability
Funding
- Fundamental Research Funds for the Central Universities
- SCUT [2011ZB0012]
- National Natural Science Foundation of China [31201330]
- Key Project for Innovation of Science and Technology of Guangdong Province Universities [2012CXZD0009]
- Project on the Integration of Industry, Education and Research of Guangdong Province and Ministry of Education [2012B091100113]
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Two novel polysaccharide fractions, named WP1 and WP3, were extracted and purified from wampee [Clausena lansium (Lour.) Skeels] seeds. Their physicochemical properties, in vitro and in vivo antioxidant activities were investigated. Results indicated that both WP1 and WP3 were acidic heteropolysaccharides with uronic acid content of 45.35% and 56.79%, respectively. The molar ratio of rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose of WP1 was 5.01:1.50:39.53:1:10.76:23.50 while WP3 was 5.76:1.73:27.27:1:4.72:8.77. Furthermore, WP3 not only exhibited stronger in vitro antioxidant activities than WP1, but also more significantly increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) level in serum of SD rats induced by high-fat diet. (C) 2013 Elsevier B.V. All rights reserved.
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