Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 62, Issue -, Pages 296-303Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2013.09.001
Keywords
Collagen; Gelatin; Renaturation; Fibril; Cell encapsulation; Three-dimensional cell culture
Funding
- JSPS KAKENHI [23791678]
- NIMS Molecule & Material Synthesis Platform in Nanotechnology Platform Project
- Grants-in-Aid for Scientific Research [23791678] Funding Source: KAKEN
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Collagen-based 3-D hydrogels often lack sufficient mechanical strength for tissue engineering. We developed a method for fabrication of high-density collagen fibril matrix (CFM) gels from concentrated solutions of uncleaved gelatin (UCG). Denatured random-coil UCG exhibited more rapid and efficient renaturation into collagen triple-helix than cleaved gelatin (CG) over a broad range of setting temperatures. The UCG solution formed opaque gels with high-density reconstituted collagen fibrils at 28-32 degrees C and transparent gels similar to CG at <25 degrees C. The unique gelation properties of UCG enabled the encapsulation of cultured cells in CFM of high solid volume (>5%) and elasticity (1.28 +/- 0.15 kPa at 5% and 4.82 +/- 0.38 kPa at 8%) with minimal cell loss. The elastic modulus of these gels was higher than that of conventional CFM containing 0.5% collagen. High-strength CFM may provide more durable hydrogels for tissue engineering and regenerative medicine. (C) 2013 Elsevier B.V. All rights reserved.
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