Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 51, Issue 5, Pages 1033-1042Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2012.08.020
Keywords
Chitosan; Natural hydroxyapatite; Chondroitin sulfate; Amylopectin; Bone tissue engineering; Marine Biomaterials
Funding
- Marine Bioprocess Research Centre of the Marine Bio 21 Center
- Ministry of Land, Transport and Maritime, Republic of Korea
- Korea Institute of Marine Science & Technology Promotion (KIMST) [20046002] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Over the past few decades, artificial graft materials for bone tissue engineering are gaining much importance. In this study, tri-component scaffolds of chitosan/natural hydroxyapatite with chondroitin sulfate (chitosan-CS/HAp) and amylopectin (chitosan-AP/HAp) have been developed for the first time via freeze-drying method and were characterized physicochemically for bone grafting substitutes. Chemical interactions and dispersion of HAp, CS and AP in the chitosan matrix have been evaluated by various analytical techniques. The porosity and water uptake/retention ability of these composite scaffolds decreased whereas thermal stability increased when compared to the chitosan scaffold. The pore size of the chitosan/HAp, chitosan-CS/HAp and chitosan-AP/HAp scaffolds varied from 60 to 180 mu m, 60 to 400 mu m and 80 to 500 mu m, respectively. Cell proliferation, alkaline phosphatase activity and type-1 collagen production was evaluated in vitro using MG-63 cell line, which was observed to be higher in the composite scaffolds. Excellent interconnected porosity, controlled biodegradation and enhanced cell proliferation of the novel chitosan-CS/HAp and chitosan-AP/HAp scaffolds suggests that these scaffolds are promising biomaterials for bone tissue engineering. (C) 2012 Elsevier B.V. All rights reserved.
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