Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 50, Issue 4, Pages 1035-1039Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2012.02.031
Keywords
Guar gum; Characterization; Enzymatic hydrolysis; Viscosity; Molecular weight
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Guar gum was hydrolyzed using cellulase from Aspergillus niger at 5.6 pH and 50 degrees C temperature. Hydrolyzed guar gum sample was characterized using Fourier transform infrared spectroscopy, differential scanning calorimetry, thermogravimetric analysis, X-ray diffraction, dilute solution viscometry and rotational viscometry. Viscometry analysis of native guar gum showed a molecular weight of 889742.06, whereas, after enzymatic hydrolysis, the resultant product had a molecular weight of 7936.5. IR spectral analysis suggests that after enzymatic hydrolysis of guar gum there was no major transformation of functional group. Thermal analysis revealed no major change in thermal behavior of hydrolyzed guar gum. It was shown that partial hydrolysis of guar gum could be achieved by inexpensive and food grade cellulase (Aspergillus niger) having commercial importance and utilization as a functional soluble dietary fiber for food industry. (C) 2012 Elsevier B.V. All rights reserved.
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