Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 51, Issue 1-2, Pages 1-6Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2012.04.024
Keywords
Hen egg white lysozyme; Fibrillation; Cu(II) reduction
Funding
- Department of Biotechnology, Government of India [BT/PR13931/MED/3]
- Department of Science and Technology, Government of India [SR/NM/NS-80/2008]
- CSIR, New Delhi
- IIT Kharagpur
Ask authors/readers for more resources
Copper is known to exert diverse effects on the self-association of proteins and has been found in amyloid deposits that are involved in neurodegenerative disease processes. The effects of the metal ion on the protein during fibrillation were investigated by fluorescence, circular dichroism spectroscopy and fluorescence microscopy. We report for the first time, the complete reduction of Cu(II) -> Cu(I) in vitro during fibrillation of hen egg white lysozyme at pH 7. This was confirmed by the lack of any signal for Cu(II) in electron paramagnetic resonance spectroscopy and quantification of Cu(I) was achieved by a bathocuproine disulfonate based assay. (c) 2012 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available