Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 48, Issue 1, Pages 165-169Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2010.10.015
Keywords
Ganoderma lucidum; Polysaccharide; Purification; NMR characterization
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A water-soluble neutral polysaccharide (GLP-F1-1) was isolated from the fruiting bodies of Ganoderma lucidum by DEAE Sepharose Fast Flow and Sephacryl S-500 High Resolution Chromatography. The neutral polysaccharide had an average molecular weight (M(w)) of approximately 2.5 x 10(6) kDa. GC analysis showed that this polysaccharide was mainly composed of glucose and galactose in the molar ratio of 34:1. (1)H and (13)C NMR spectroscopy in combination with GC-MS technique indicated that the new polysaccharide had a backbone chain of 1,4-disubstituted-beta-glucoseopyranose and 1,4,6-trisubstituted-beta-glucoseopyranosyl, while the branched chains were mainly composed of 1,6-disubstituted-beta-glucopyranosyl and 1,4-disubstituted-beta-galactoseopyranosyl residues. (C) 2010 Elsevier B.V. All rights reserved.
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