Purification and structural characterization of a new water-soluble neutral polysaccharide GLP-F1-1 from Ganoderma lucidum

A water-soluble neutral polysaccharide (GLP-F1-1) was isolated from the fruiting bodies of Ganoderma lucidum by DEAE Sepharose Fast Flow and Sephacryl S-500 High Resolution Chromatography. The neutral polysaccharide had an average molecular weight (M(w)) of approximately 2.5 x 10(6) kDa. GC analysis showed that this polysaccharide was mainly composed of glucose and galactose in the molar ratio of 34:1. (1)H and (13)C NMR spectroscopy in combination with GC-MS technique indicated that the new polysaccharide had a backbone chain of 1,4-disubstituted-beta-glucoseopyranose and 1,4,6-trisubstituted-beta-glucoseopyranosyl, while the branched chains were mainly composed of 1,6-disubstituted-beta-glucopyranosyl and 1,4-disubstituted-beta-galactoseopyranosyl residues. (C) 2010 Elsevier B.V. All rights reserved.