Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 48, Issue 1, Pages 13-19Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2010.09.019
Keywords
Chitosan-graft-poly (epsilon-caprolactone)/poly (epsilon-caprolactone) nanofibrous scaffolds; Chitosan-graft-poly (epsilon-caprolactone); Skin tissue engineering; Electrospin; Cationic nanofibrous mats
Funding
- National Natural Science Foundation of China [50843031]
- Shanghai Municipality Commission for Special Project of Nanometer Science and Technology [0952nm05300]
- Shanghai Municipality Commission for Non-governmental International Corporation Project [09540709000]
- Shanghai Municipality Commission [10410710000]
- Fundamental Research Funds for the Central Universities [WUT 2010-II-022]
- Science and Technology Commission of Shanghai [09PJ1407200]
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This research is aimed to develop cationic nanofibrous mats with improved cellular adhesion profiles and stability of three-dimensional fibrous structure as potential scaffolds for skin tissue engineering. Firstly, amino-remained chitosan-graft-poly (epsilon-caprolactone)(CS-g-PCL) was synthesized with a facile one-step manner by grafting epsilon-caprolactone oligomers onto the hydroxyl groups of CS via ring-opening polymerization by using methanesulfonic acid as solvent and catalyst. And then, CS-g-PCL/PCL nanofibrous mats were obtained by electrospinning of CS-g-PCL/PCL mixed solution. Scanning electron microscopy (SEM) images showed that the morphologies and diameters of the nanofibers were mainly affected by the weight ratio of CS-g-PCL to PCL The enrichment of amino groups on the nanofiber surface was confirmed by X-ray photoelectron spectroscopy (XPS). With the increase of CS-g-PCL in CS-g-PCL/PCL nanofiber, the content of amino groups on the nanofiber surface increased, which resulted in the increase of zeta-potential of nanofibers. Studies on cell-scaffold interaction were carried out by culturing mouse fibroblast cells (L929) on CS-g-PCL/PCL nanofibrous mats with various contents of CS-g-PCL by assessing the growth, proliferation and morphologies of cells. The results of MTS assay and SEM observation showed that CS-g-PCL/PCL (2/8) mats with a moderate surface zeta-potential (zeta = 3 mV) were the best in promoting the cell attachment and proliferation. Toluidine blue staining further confirmed that L929 cells grew well and exhibited a normal morphology on the CS-g-PCL/PCL (2/8) mats. These results suggested the potential utilization of CS-g-PCL/PCL (2/8) nanofibrous mats for skin tissue engineering. (C) 2010 Elsevier B.V. All rights reserved.
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