Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 49, Issue 4, Pages 752-760Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2011.07.010
Keywords
Cytochrome; Folding; Intermediate; Sucrose; Phosphate
Funding
- Department of Science and Technology (DST), Government of India
- Council of Scientific and Industrial Research (CSIR), Government of India
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Spectroscopic and calorimetric investigations of the folding of denatured cytochrome c in the presence of phosphate ion and sugar were carried out to understand subtle differences in the nature of induced conformation and folding energy landscape. Altered conformations of cyt c induced by sucrose and phosphate, with same absorbance wavelength maxima, exhibit lack of tertiary interactions in segment 70-85 and similar a-helical content. However, compactness, the exposure of the heme to solvent and the secondary structure content in the two conformations are different. Although downhill folding was observed for both conformations, extent of cooperativity is higher in case of phosphate-induced conformation. (C) 2011 Elsevier B.V. All rights reserved.
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