Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 47, Issue 2, Pages 283-287Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2010.04.007
Keywords
Biodegradable; Polyhydroxyalkanoate; Biopolymer; Marine bacteria; Biodiesel byproduct; Jatropha curcas
Funding
- CSIR
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PHAs are biodegradable and environmentally friendly thermoplastics. The major contributor to PHA production cost is carbon substrate cost, therefore it is desirable to produce PHA from waste/byproducts like Jatropha biodiesel byproducts. This study was done using Jatropha biodiesel byproduct as carbon source, to decrease production cost for PHAs. Total 41 isolates from soil and marine source were able to utilize Jatropha biodiesel byproduct. Nine bacteria were selected for further studies, which were found positive for Nile red viable colony screening. Two bacterial isolates SM-P-1S and SM-P-3M isolated from soil and marine environment respectively, were found promising for PHA production. PHA accumulation for SM-P-1S and SM-P-3M was 71.8% and 75% PHA/CDW respectively and identified as Bacillus sonorensis and Halomonas hydrothermalis by MTCC. The PHA obtained from SM-P-1S and SM-P-3M was analyzed by FTIR and NMR as polyhydroxybutyrate (PHB). (C) 2010 Elsevier B.V. All rights reserved.
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