Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 47, Issue 3, Pages 361-365Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2010.05.020
Keywords
Lysozyme; Ag colloids; SERS
Funding
- DRDO, India
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In this article, we discuss metal-protein interactions in the Ag-lysozyme complex by spectroscopic measurements. The analysis of the variation in relative intensities of SERS bands reveals the orientation and the change in conformation of the protein molecules on the Ag surface with time. The interaction kinetics of metal-protein complexes has been analyzed over a period of 3 h via Raman measurements. Our analysis indicates that the Ag nanoparticles most likely interact with Trp123 which is in close proximity to Phe34 of the lysozyme molecule. (C) 2010 Elsevier B.V. All rights reserved.
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