Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 47, Issue 2, Pages 244-249Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2010.03.026
Keywords
Tea polysaccharide (TPS); Molar masses; Polydispersity; Conformation; Viscosimetry; HPSEC-MALLS-RI
Funding
- Natural Science Foundation of China [20776002]
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Tea polysaccharide (TPS) is attracting more attention gradually due to its particular biological properties. However, molecular characteristics of TPS are unclear since appropriate method is still absent. So, study on the molecular characteristics of TPS was carried out by high-performance size-exclusion chromatography (HPSEC), multi-angle laser light scattering (MALLS) and viscosimetry. The results showed that the molar masses of TPS ranged from 2.287 x 10(5) to 2.762 x 10(5) g mol(-1), the RMS radii distributed from 132.1 to 145.9 nm, and M-w/M-n is 1.028. The Mark-Houwink equation was established as [eta] = 0.5423(w)(0.5379), and the intrinsic viscosity and molecular chain parameters were as follows: [eta] = 1.007 dLg(-1), k(H) = 0.845, k(K) = 0.387, alpha = 0.5379, K= 0.5423.In addition, based on the slope of the RMS radius versus molar mass conformational plot being 0.24 +/- 0.00, we suggest that the molecular morphology of TPS is a homogeneous and spherical polymer with branch in solution. (C) 2010 Elsevier B.V. All rights reserved.
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